Journal: Nature Communications

Article Title: Radial glia integrin avb8 regulates cell autonomous microglial TGFβ1 signaling that is necessary for microglial identity

doi: 10.1038/s41467-025-57684-y

Figure Lengend Snippet: Mouse Line and Reagent List

Article Snippet: Antibody , EGFP , Origene , Goat polyclonal antibody. Cat#: R1091P. RRID:AB_1002036. , Used at 1:2000..

Techniques: RNAscope, Multiplex Assay

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Aging-Associated miR-217 Aggravates Atherosclerosis and Promotes Cardiovascular Dysfunction

doi: 10.1161/atvbaha.120.314333

Figure Lengend Snippet: Figure 5. Inhibition of endogenous miR-217 improves vascular contractile function and reduces atherosclerosis development. A, miR-217-Sponge (miR-217SPG) construct used for vascular miR-217 inhibition. Four miR-217 complementary sites, separated by 4-nt spacers, were placed downstream of GFP in the pHRSIN lentiviral vector. B, CD31 and GFP immunostaining in ascending aorta sections of apoE−/− mice inoculated through the jugular vein with control or miR-217SPG pHRSIN lentivirus. C, Concentration-response curves to acetylcholine (left) and the NO donor diethylamine NONOate (DEA-NO; right) and (D) effect of L-NAME on the concentration-response curve to phenylephrine (Phe), in aortic segments from male control and miR-217SPG–infected apoE−/− mice fed HFD for 12 wk. E, Red oil staining analysis of atherosclerotic lesion area in aortic sinus and ascending aorta sections from control and miR-217SPG–infected apoE−/− mice fed an HFD for 12 wk. Left, Representative red oil–stained cross sections 320 µm from the aortic sinus in control and miR-217SPG–infected mice. *P<0.05 by unpaired t test or ANOVA.

Article Snippet: Aortic cross sections were stained with antibodies against GFP (Acris Antibodies) and CD31 (Abcam).

Techniques: Inhibition, Construct, Plasmid Preparation, Immunostaining, Control, Concentration Assay, Infection, Staining

Journal: Iranian Biomedical Journal

Article Title: Leishmania Parasite: the Impact of New Serum-Free Medium as an Alternative for Fetal Bovine Serum

doi: 10.52547/ibj.25.5.349

Figure Lengend Snippet: Determination of EGFP expression in CSMF medium in comparison with M199 supplemented with 5% FBS. (A) Assessment of EGFP expression by epifluorescence microscopy, indicating the EGFP expression of L. tarentolae -EGFP and L. tarentolae -PpSP15-EGFP promastigotes in the logarithmic and stationary phases in both CSFM and M199 5% FBS media. (b) Flow cytometry analysis of recombinant L. tarentolae -PpSP15-EGFP (right panel), L. tarentolae EGFP as a positive control (middle panel), and the L. tarentolae wild type as a negative control (left panel) using a FITC detector in CSFM and M199 5% FBS at logarithmic (upper panel) and stationary (lower panel) phases. (C) Western blot analysis to confirm the expression of PpSP15-EGFP by recombinant L. tarentolae -PpSP15-EGFP parasite in CSFM and M199 with 5% FBS using an anti-GFP antibody. A 42-kDa band relating to the expression of PpSP15-EGFP protein in concentrated supernatant of L. tarentolae -PpSP15-EGFP parasite was detected in M199 with 5% FBS (lanes 2 and 3) and CSFM medium (lanes 5 and 6), respectively at logarithmic and stationary phases. A 27-kDa band indicating EGFP protein in the L. tarentolae -EGFP parasite (lane 1) and wild type form of L. tarentolae parasite as negative control was cultivated in M199 with 5% FBS (lane 4)

Article Snippet: Acrylamide and anti-GFP-HRP goat polyclonal antibody were procured from Merck and Acris Antibodies GmbH (Herford, Germany), respectively.

Techniques: Expressing, Comparison, Epifluorescence Microscopy, Flow Cytometry, Recombinant, Positive Control, Negative Control, Western Blot